ASIAN PACIFIC JOURNAL OF ALLERGY AND IMMUNOLOGY (2005) 23: 205-211 Evaluation of Human IgG Subclass Anti- bodies in the Serodiagnosis of Para- gonimiasis Heterotremus
نویسندگان
چکیده
SUMMARRY Immunoglobulin G subclass antibodies (IgG1, IgG2, IgG3, and IgG4) responses to the excretorysecretory antigens of the lung fluke, Paragonimus heterotremus, were analyzed using the immunoblotting technique in an attempt to further improve the sensitivity and specificity for serodiagnosis of human paragonimiasis. Serum samples from patients with proven paragonimiasis, from patients with other parasitic infections, pulmonary tuberculosis and from healthy counterparts were analyzed. The results indicate that immunoblotting for the detection of IgG4 antibodies to an excretory-secretory product of P. heterotremus of an approximate molecular mass of 31.5 kDa, is the most reliable test. It gives accuracy, sensitivity, specificity, and positive and negative predictive values of 97.6%, 100%, 96.9%, 90% and 100%, respectively.
منابع مشابه
EVALUATION OF SERUM IgG SUBCLASS LEVELS IN ASTHMATIC AND ATOPIC CHILDREN WITH RECURRENT INFECTION
Serum IgG subclass levels were measured using an indirect immunoenzymatic assay (ELISA) with monoclonal antibodies in 16 children with asthma and 13 children with atopy who had mostly recurrent infections. Seven of the asthmatic children had marked low or low normal levels of IgG4, six had marked low or low normal levels of IgG 3, two had marked low normal levels of both IgG2, and IgG 3 and...
متن کاملAnticardiolipin Antibodies in Juvenile Rheumatoid Arthritis and Systemic Lupus Erythematosus
Background: Antiphospholipid antibody syndrome (APS) can either occur as a primary syndrome or associated with other autoimmune diseases such as systemic lupus erythematosus (SLE). Anticardiolipin antibody (aCL) of IgG and/or IgM isotype in blood, measured by a standardized ELISA is the most acceptable laboratory criteria. APS IgG isotype, particularly IgG2 subclass is more strongly associated ...
متن کاملImmunoglobulin values in healthy Thai children aged ≤ 24 months determined by nephelometry.
BACKGROUND Variation of normal immunoglobulin (Ig) levels between different genetic and environment factors has been studied. Although antibody deficiency diseases can start from infancy, data of Ig reference levels in children aged ≤24 months are still limited, especially in Asian children. PURPOSE The aim of this study was to determine serum IgG, IgA, IgM, and IgG subclasses in healthy Thai...
متن کاملImmunoglobulin G (IgG) subclass deficiency in Thai children.
BACKGROUND Patients with Immunoglobulin G (IgG) subclass deficiency may suffer from recurrent infections, mainly sino-pulmonary infection. OBJECTIVE To determine the epidemiology of IgG subclass deficiency in Thai children at a tertiary care hospital and to compare the differences between children who were diagnosed with IgG subclass deficiency by using low level criteria [less than 2 standar...
متن کاملAllergic rhinitis and immunoglobulin deficiency in preschool children with frequent upper respiratory illness.
BACKGROUND Frequent upper respiratory illness (URI) is a common problem in preschool children. Allergic rhinitis and immunoglobulin (Ig) deficiency are usually suspected as underlying etiologies. OBJECTIVE To determine the prevalence of allergic rhinitis and Ig and IgG subclass deficiency in preschool children with frequent URI. METHODS Two thousand eight hundred and seventy-six questionnai...
متن کامل